Beer-Lambert Law Calculator
Calculate absorbance, concentration, molar extinction coefficient, or path length using the Beer-Lambert Law equation: A = ε × c × l
Disclaimer: This calculator is for informational and educational purposes only. Results are estimates and should be verified independently. No liability is assumed for errors or outcomes resulting from its use.
Calculation Results
Understanding the Beer-Lambert Law
The Beer-Lambert Law (also known as Beer's Law) is a foundational principle in spectroscopy and analytical chemistry, relating the attenuation of light to the properties of the material through which the light is traveling. The mathematical relationship is:
A = ε × c × l
Where:
- A = Absorbance (dimensionless)
- ε = Molar extinction coefficient or molar absorptivity (L/(mol·cm))
- c = Concentration of the absorbing species (mol/L)
- l = Path length of the sample the light passes through (cm)
Applications of the Beer-Lambert Law Calculator
Our Beer-Lambert Law Calculator is an essential tool for scientists, researchers, and students working in:
- Analytical Chemistry - Quantitative determination of solution concentrations
- Biochemistry - Protein and nucleic acid quantification
- Environmental Science - Water quality analysis and pollutant detection
- Pharmaceutical Research - Drug formulation and quality control
- Clinical Laboratories - Diagnostic testing and blood analysis
- Food Science - Analysis of food components and additives
How to Use This Calculator
- Select which variable you want to calculate from the dropdown menu
- Enter the known values in the appropriate fields
- Click "Calculate" to instantly get your result
- Review the detailed explanation of the calculation
Important Considerations
When using the Beer-Lambert Law, remember these key points:
- The law is valid only for dilute solutions (typically absorbance values below 1.0)
- The relationship is linear at lower concentrations but may deviate at higher concentrations
- The absorbing species should not participate in concentration-dependent reactions
- The incident light should be monochromatic (single wavelength)
- The solution should be homogeneous without scattering particles
Our free Beer-Lambert Law Calculator provides a quick and accurate way to perform spectroscopy calculations without complex manual computations!